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Old 11-20-2006, 01:01 PM   #1
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Default Trying to pull a Fast Times at Ridgemont High

I work in a college science lab, the other day we where running a PCR lab where part of the experiment takes about 45 minutes, during this time some of the students decided to order out for some pizza and soda. While i was standing just outside the lab eating my lunch i saw the Domino's guy walk into the building and right into the classroom. I couldn't believe that these kids would actually think that some shit like that was going to fly.

We kicked them out of the lab and they all received "0" for the week. It was sweet to see their face. I bet their food didn't taste as good after that.

Ha, Ha. What a pack of idiots.
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Old 11-20-2006, 01:04 PM   #2
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Quote:
Originally Posted by deadhead13
I work in a college science lab, the other day we where running a PCR lab where part of the experiment takes about 45 minutes, during this time some of the students decided to order out for some pizza and soda. While i was standing just outside the lab eating my lunch i saw the Domino's guy walk into the building and right into the classroom. I couldn't believe that these kids would actually think that some shit like that was going to fly.

We kicked them out of the lab and they all received "0" for the week. It was sweet to see their face. I bet their food didn't taste as good after that.

Ha, Ha. What a pack of idiots.
Eating in the lab is very, very against state/federal guidelines. And what kind of PCR reaction were you doing? Amplification or mutagenesis?

*EDIT*

Nevermind.. no way you can run a mutagenesis in 45 minutes. Sounds like small oligonucleotide amplification considering the extension time had to have been under a few minutes.

Last edited by badmotherfarker; 11-20-2006 at 01:06 PM..
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Old 11-20-2006, 01:06 PM   #3
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Quote:
Originally Posted by badmotherfarker
Eating in the lab is very, very against state guidlines. And what kind of PCR reaction were you doing? Amplification or mutagenesis?
Amplification, althought that particular lab they are just learning how to work with some of the how to techniques. It's a General Bio. for mayors.

the timing for that part of the experiment for them is about 45min to an hour.
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Old 11-20-2006, 01:10 PM   #4
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2.5 uL 10X Taq buffer
2 uL MgCl2
2 uL plasmid template
0.5 uL 5' primer
0.5 uL 3' primer
0.5 uL Taq polymerase
17 uL ddH2O

... I think I spent 50% of my PhD writing that into my notebook.
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Old 11-20-2006, 01:49 PM   #5
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this is some excellent iced tea.
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Old 11-20-2006, 01:50 PM   #6
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Instead of Mr Hand, we've got Mr. head...

Damn kids these days.
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Old 11-20-2006, 10:10 PM   #7
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Quote:
Originally Posted by deadhead13
It's a General Bio. for mayors.

And I always thought politicians were stupid...
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Old 11-21-2006, 06:44 AM   #8
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Quote:
Originally Posted by dantodd
And I always thought politicians were stupid...
Right, I meant majors.
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Old 11-21-2006, 06:54 AM   #9
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I am so far removed from the world of academics that I read your first post and thought "not a bad idea, lab time was always sucky."

Went to PSU, graduated and instatnly forgot all the crap that was outside my major. I say, bring the Pizza's to a lab, who cares.

"learning about Cuba, havin some food"
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Old 11-22-2006, 12:43 PM   #10
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Aside from food and drink in the lab being big no-no's in terms of personal safety and regulations, labs where PCR is being done have to be kept very VERY clean, to keep from contaminating reagents and equipment with DNA and nucleases and the like, not to mention pepperoni grease.
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Old 11-22-2006, 12:46 PM   #11
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Quote:
Originally Posted by badmotherfarker
Eating in the lab is very, very against state/federal guidelines. And what kind of PCR reaction were you doing? Amplification or mutagenesis?

*EDIT*

Nevermind.. no way you can run a mutagenesis in 45 minutes. Sounds like small oligonucleotide amplification considering the extension time had to have been under a few minutes.
Stratagene's "Quikchange" kit has revolutionized the science of mutagenesis. What used to take weeks of reactions and screening now only takes a couple of days to get reliable mutant clones.
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Old 11-22-2006, 05:08 PM   #12
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Quote:
Originally Posted by deadhead13
It was sweet to see their face.
I agree with the lab not being the best place for food and drink. But are you that hard up for amusement?
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Old 11-22-2006, 05:22 PM   #13
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Quote:
Originally Posted by RyeGuy
Stratagene's "Quikchange" kit has revolutionized the science of mutagenesis. What used to take weeks of reactions and screening now only takes a couple of days to get reliable mutant clones.
Been using Stratagene Quickchange for 5 years now- not really 'revolutionary' anymore (and you can actually get clones plus sequences back within 24 hrs). Mutagenesis on any plasmid over 6.5 kB still takes 6-8 hrs though, so, although it's faster, it's not all that fast. Any reaction that is under an hour is sub 100 bp amplification.

Last edited by badmotherfarker; 11-22-2006 at 05:30 PM..
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Old 11-27-2006, 03:05 AM   #14
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Quote:
Originally Posted by RyeGuy
Stratagene's "Quikchange" kit has revolutionized the science of mutagenesis. What used to take weeks of reactions and screening now only takes a couple of days to get reliable mutant clones.
"I think I'm dumb"
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Old 11-28-2006, 12:22 AM   #15
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Quote:
Originally Posted by siriusurfer
"I think I'm dumb"
Nah, just a different knowledge base. Everyone has areas of expertise.

Unless they're dumb...
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